Skin diseases such as wound healing, acne, dermatitis, psoriasis, irritated skin, sunburn, infections, etc., are associated with inflammation. Drugs and treatments for skin diseases can cause inflammation. Combining topical delivery of H2 with standard therapies for skin conditions should reduce inflammation. H2B* facilitates sustained topical delivery of H2 and drugs. Two Examples are as follows:
Example 1: Anti-acne H2 Formulation
An anti-acne, keratolytic preparation consisting of H2B* and 2% salicylic acid, in distilled water was prepared. After expansion, the formulation was found to contain 25.4% of H2. Sites were delineated on the skin of the forearm. The formulation was spread uniformly over the test site. The concentration of H2 being delivered to the skin surface was determined. Salicylic acid on the skin surface and in the stratum corneum was observed with a Wood’s Light (UVA). Tape stripping the skin on the site allows for determining the layers of stratum corneum penetrated by salicylic acid. It was found that the H2B* formulation delivered salicylic acid deeply - through 9-10 layers of stratum corneum.
Immediately after application, it was determined that 432 ppb H2 was in contact with the skin surface. Within 10 minutes this value dropped to 250 ppb. At 20 minutes it was 167 ppb. At 30 minutes, it had dropped to zero. A substantial portion of the H2 in contact with the skin surface was absorbed into the skin. The stratum corneum, the top barrier of the skin, is hydrophobic. H2 is also hydrophobic. Hydrophobic entities associate with each other, in an aqueous environment.
Example 2: H2 Moisturizing Lotion for Dry, Aging skin
As skin ages from environmental damage, it is subject to sub-chronic inflammation affecting loss of hydroscopic macromolecules – thus, reducing its moisture holding capacity. A preparation that will reduce sub chronic inflammation - and moisturize, should slow down skin aging. By sustained topical delivery of H2, H2B* makes this possible. A H2-generating moisturizing lotion was prepared: 1.6% H2B*, 3% lactic acid, 20% glycerin, 78.4% distilled water. The lotion released 815 ppb H2. The site was treated topically with 0.15 g./square cm. of the lotion. Immediately after treatment, the H2 on the skin surface was 804 ppb. H2 fluctuated and did not show a progressive loss up to 60 minutes. H2 measurements showed the presence of significant H2 on the skin surface - up to 210 minutes. This persistence of H2 on the skin surface was surprising. The lotion rapidly loses most of its water as it dries on the skin surface. Such persistence may be explained by:
1) H2B* deposited on the skin surface, continues to react with water held on the skin surface by the hydroscopic effects of glycerin and lactic acid;
2) The stratum corneum has a reservoir effect for hydrophobic molecules. H2 is hydrophobic. It may contribute to the H2 detected on the skin surface due to back diffusion of H2 from the stratum corneum.