H2 promotes the health and growth of both animal and plant life. Aquatic life in the form of plants, invertebrates (e.g., shrimp, crabs, etc.) and fish should also benefit from the anti-oxidant and enhanced bioavailability effects of H2. H2 reduces reactive oxygen species (ROS). ROS affect the consumption of energy that could otherwise be used for growth and fighting disease.
Example 1: Sustained Delivery of H2 to an Aquarium or Fish Farm
An experiment was designed to determine if an H2B* formulation, that generates H2, can deliver and sustain H2 in a model aquatic farm. Phosphate buffered saline (PBS) in distilled water(DW) was prepared, pH 7.6. Also, a formulation of 4.1% H2B* and 0.5 % ascorbic acid in DW – was prepared. The H2 released was determined to be 1.5 ppm.
The 125 mL formulation was placed in the 1,000 mL PBS solution. Being less dense than PBS, it floated on top of the 1,000 mL container. The PBS was constantly circulated.
At baseline, there was 0 ppb H2 in the PBS solution, pH of 7.6. After 15 minutes, the H2 in the PBS remained at 0 ppb, pH of 7.6. By 4.7 hours, there was 562 ppb H2 in the PBS, pH of 7.7. At 13.8 hours, H2 in the PBS had risen to 691 ppb, pH of 7.7. At 28.3 hours, the H2 in the PBS had risen to 1245 ppb, pH 7.6. Thereafter, H2 in the PBS was sustained at a level of 1202 ppb, pH 7.3 until 109 hours. At 123 hours there was 1165 ppb H2, pH 6.8. The pH drop probably indicates bacterial growth in the simulated tank. This experiment shows the feasibility of delivering H2 to an aquarium or aquatic farm. It also points to routine steps that must be taken to insure the maintenance of water quality